Application of transmission electron microscopy and UV-microspectrophotometry for detection of lignin in cell wall of conifers

Jožica Gričar and Katarina Čufar (2004) Application of transmission electron microscopy and UV-microspectrophotometry for detection of lignin in cell wall of conifers. Zbornik gozdarstva in lesarstva (73). pp. 89-104. ISSN 0351-3114

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Abstract

Potassium permanganate represents a useful tool in transmission electron microscopy (TEM) for the localization of lignin in various morphological regions of wood. Since this method only provides qualitative estimation, another quantitative method is recommended to verify the results obtained by TEM, such as UV-microspectrophotometry (UMSP). The UMSP method was found to besuited for the determination of lignin content and its distribution in woodytissue. The present paper provides a methodical description and presents the applicability of TEM and UMSP for studies in the topochemistry of lignin distribution within cell walls.

Item Type:	Article
Keywords:	UV-microspectrophotometry, transmission electron microscopy, potassium permanaganate, light microscopy, lignin, cell wall, wood, conifers
Link to COBISS:	http://www.cobiss.si/scripts/cobiss?command=SEARCH&base=COBIB&select=ID= 1268390
Divisions:	Biotechnical Faculty, Department of Wood Technology > Chair of wood science Slovenian Forestry Institute > Department of Forest Yield and Silviculture
Item ID:	262
Date Deposited:	26 Feb 2014 15:31
Last Modified:	26 Feb 2014 15:31
URI:	http://eprints.gozdis.si/id/eprint/262

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